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13683 1 ap rabbit polyclonal  (Proteintech)


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    Structured Review

    Proteintech 13683 1 ap rabbit polyclonal
    13683 1 Ap Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13683+1+ap+rabbit+polyclonal/CHMP4B+Polyclonal+antibody/pmc12542950__sciadv%2Eadv6805_sm-97-36-34
    Average 94 stars, based on 91 article reviews
    13683 1 ap rabbit polyclonal - by Bioz Stars, 2026-09
    94/100 stars

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    Article Title: Lysosomal damage is a therapeutic target in Duchenne muscular dystrophy
    Article Snippet: Antibody Supplier / Reference Species Dilution Anti-ALIX Santacruz, sc-53540 Mouse monoclonal IgG1 1:50 Anti-CTSB Cell Signaling, 31718 Rabbit monoclonal 1:400 Anti-CTSD Abcam, ab75852 Rabbit monoclonal 1:100 Anti-CD11B BD Pharmingen, 550282 Rat monoclonal 1:40 Anti-CHMP4B ProteinTech group, 13683-1-AP Rabbit polyclonal 1:200 Anti-Dystrophin Leica, DYSB Mouse monoclonal IgG1 1:50 Anti-LAMP2 Abcam, Ab13524 Rat monoclonal 1:200 Anti-LAMININ Abcam, ab11576 Rat monoclonal 1:200 Anti-LAMININ Invitrogen, PA1-1673 Rabbit polyclonal 1:500 Anti-LC3B Sigma, L7543 Rabbit polyclonal 1:100 Anti-LGALS3 Santacruz, Sc-32790 Mouse monoclonal IgG1 1:50 Anti-SGCG Abcam, ab203112 Rabbit Monoclonal 1:200 Anti-SQSTM1 Sigma, P0067 Rabbit polyclonal 1:100 Anti-TFEB ThermoFisher Scientific, A303673A Rabbit polyclonal 1:100



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    Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against <t>CHMP4B</t> of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.
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    Proteintech rabbit polyclonal anti chmp4b
    Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against <t>CHMP4B</t> of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.
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    Image Search Results


    Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against CHMP4B of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.

    Journal: The Journal of cell biology

    Article Title: Vps60 initiates alternative ESCRT-III filaments.

    doi: 10.1083/jcb.202206028

    Figure Lengend Snippet: Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against CHMP4B of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.

    Article Snippet: Rabbit monoclonal antibody against LAMP1 (1/1,000 dilution for immunofluorescence) was from Cell Signaling (9091), and rabbit polyclonal antibody against CHMP4B (1/300) was from Proteintech (13683-1-AP).

    Techniques: Activity Assay, Immunofluorescence, Staining, Expressing, Live Cell Imaging

    Figure 8. CHMP5 and CHMP4B polymers have distinct dynamics in cells. (A) Decon- voluted confocal images of immunofluores- cence staining against LAMP1 or CHMP4B (same as Fig. 7 C, higher magnification) of Hela- Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment (scale bar: 10 μm, 2 μm inlet). (B) Live-cell imaging of Hela-Kyoto cells expressing CHMP4B-GFP and CHMP5- mScarlet-I undergoing nuclear envelop refor- mation and cytokinetic abscission (scale bar: 10 μm). Arrowheads indicate the reforming nu- clear envelope and the cytokinetic bridge. (C) Cartoon of the proposed model for function of Vps60-based ESCRT-III filaments.

    Journal: The Journal of cell biology

    Article Title: Vps60 initiates alternative ESCRT-III filaments.

    doi: 10.1083/jcb.202206028

    Figure Lengend Snippet: Figure 8. CHMP5 and CHMP4B polymers have distinct dynamics in cells. (A) Decon- voluted confocal images of immunofluores- cence staining against LAMP1 or CHMP4B (same as Fig. 7 C, higher magnification) of Hela- Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment (scale bar: 10 μm, 2 μm inlet). (B) Live-cell imaging of Hela-Kyoto cells expressing CHMP4B-GFP and CHMP5- mScarlet-I undergoing nuclear envelop refor- mation and cytokinetic abscission (scale bar: 10 μm). Arrowheads indicate the reforming nu- clear envelope and the cytokinetic bridge. (C) Cartoon of the proposed model for function of Vps60-based ESCRT-III filaments.

    Article Snippet: Rabbit monoclonal antibody against LAMP1 (1/1,000 dilution for immunofluorescence) was from Cell Signaling (9091), and rabbit polyclonal antibody against CHMP4B (1/300) was from Proteintech (13683-1-AP).

    Techniques: Staining, Expressing, Live Cell Imaging